Workshop 2: R and RStudio

R and RStudio In this online workshop you will learn the R programming language, RStudio interface for programming in R, and useful tips for exploring and working with data.

You are expected to study the the following content:

• Introduction and Data Structures

• Exploring Data in R

Here is a link to a very useful Base R Cheat Sheet that is good to keep handy (maybe you can have a printed version for yourself).

In the workshop, we will work with RNA-Seq data and perform differential analysis.

• R Workshop

Tutorials

• R and RStudio: Introduction and Data Structures
  • Getting Started
  • Basic Operations in R
  • Conditional Statements and Looping
• Exploring Data in R
  • R packages and libraries
  • Loading Data
  • Data Exploration
• R Workshop
  • Load Packages
  • Import Airway Data
  • Working with data.frame objects
  • Asking R for help
  • Working with matrix objects
  • Running simple comparative statistical analyses
  • Running one test
  • Wrapper functions
  • The apply() Family of Functions
  • Matrix operations
  • Loading data from R packages
  • Explore Airway Dataset
  • Differential Expression Analysis using DESeq2
  • Manipulate and Visualize Results
  • Gene Set Enrichment using enrichR